At this point, each bound sequence exists as a single copy, which produces too faint a signal to detect reliably. Colony sequencing solves this by generating clusters of identical fragments through a process called bridge amplification. The adapter on the free end of each DNA strand will be complementary to some of the original, short sequences on the DNA lawn, and when this binding occurs, the strand bends over to form a bridge shape. Another round of amplification takes place, resulting in two complementary strands each directly affixed to the flow cell. This “bridge amplification” process is repeated over and over to propagate the sequence.
For the web version’s accuracy, I tested different feature limits. I prioritized performance and kept 500k features—stored as JSON, it’s 107MB (though gzip on server reduces it to ~38MB). I tried smaller versions (50k, 80k)—accuracy only dropped 3–4%, but final AI detection rates varied significantly, especially for human texts, with relative errors up to ±50%, leading to false positives. So I stuck with 500k.。clash下载 - clash官方网站对此有专业解读
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